Spring 2021

ECE 564 Modern Light Microscopy

Prof. Gabriel Popescu

 

Text: “Introduction to Optical Microscopy” by J. Mertz (Roberts and Company, 2010)

“Quantitative phase imaging of cells and tissues” by G. Popescu (McGraw-Hill, 2011)

Schedule: 11:00-12:20 T-R;

Office Hours: Thursday 12:30-2:00

Teaching Assistant: Neha Goswami (email)

TA Office Hours: Friday 3:00-4:00pm

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Research Projects

#

Day

Date

Topic

Supporting Material

Homework

Links

References

28

T

Jan 26

Introduction

Microscopy Milestones (Nature)

ECE 460

27

R

Jan 28

Groundwork: 2D, 3D Fourier transforms

Groundwork

ImageJ

Papoulis [1], Bracewell [2], Goodman [3], Popescu [4]

26

T

Feb 02

Groundwork: 2D, 3D Fourier transforms

 

25

R

Feb 04

Fourier Optics

Microscopy

 

 

Popescu [5]

24

T

Feb 09

Fourier Optics

 

 

 

23

R

Feb 11

Light Microscopy

HW1 Due 

 

 

22

T

Feb 16

Holography

Holography

 

 

 

21

R

Feb 18

Quantitative phase imaging (QPI)

 

 

 

20

T

Feb 23

Quantitative phase imaging (QPI)

 

19

R

Feb 25

Propagation in inhomogeneous media

Light scattering

 

 

Born & Wolf [6], van de Hulst [7], Ishimaru [8]

18

T

Mar 02

Propagation in inhomogeneous media

 

 

 

17

R

Mar 04

Dynamic light scattering

Dynamic Light Scattering

 

Berne & Pecora [9]

16

T

Mar 09

Dynamic light scattering

 

15

R

Mar 11

Propagation of Gaussian beams

Gaussian beams

 

Yariv- Optical waves in crystals [10]

14

T

Mar 16

Propagation of Gaussian beams

 

 

 

13

R

Mar 18

Confocal microscopy

Confocal Microscopy

 

 

 

12

T

Mar 23

Review

 

 

R

Mar 25

Midterm

 

 

11

T

Mar 30

Optical coherence tomography

OCT

 

 

 

10

R

Apr 01

Optical coherence tomography

 

 

 

 

9

T

Apr 06

Propagation in nonlinear media

Nonlinear

 

 

Bloembergen [11], Boyd [12], Shen [13]

8

R

Apr 08

Propagation in nonlinear media

 

T

Apr 13

Day off

 

7

R

Apr 15

Nonlinear microscopy

Masters and So [14]

6

T

Apr 20

Nonlinear microscopy

 

 

 

5

R

Apr 22

Superresolution microscopy

Research Projects

 

 

 4

T

Apr 27

Student TALKS

Presentation Template

 

 

R

Apr 29

Student TALKS

Peer evaluation form

 

2

T

May 04

Student TALKS

 

 

R

May 06

Reading Day

 

1

M

May 10

Student TALKS

 

 

M

May 10

Reports due by midnight May 10. Upload to your Box folder

Report Template.

 

 

Grading formula: Midterm 30%;  Final report - 20%;  Homework - 30%;  Presentation 20%; Class participation - 5% .

 

REFERENCES

1.         A. Papoulis The Fourier integral and its applications (McGraw-Hill, New York,, 1962).

2.         R. N. Bracewell The Fourier transform and its applications (McGraw Hill, Boston, 2000).

3.         J. W. Goodman Introduction to Fourier optics (McGraw-Hill, New York, 1996).

4.         G. Popescu Principles of Biophotonics. Volume 1- Linear systems and the Fourier transform in optics (IOP Publishing, 2018).

5.         G. Popescu Quantitative phase imaging of cells and tissues (McGraw-Hill, New York, 2011).

6.         M. Born and E. Wolf Principles of optics : electromagnetic theory of propagation, interference and diffraction of light (Cambridge University Press, Cambridge ; New York, 1999).

7.         H. C. van de Hulst Light Scattering by Small Particles (Dover Publications New York, 1981).

8.         A. Ishimaru Electromagnetic wave propagation, radiation, and scattering (Prentice Hall, Englewood Cliffs, N.J., 1991).

9.         B. J. Berne and R. Pecora Dynamic Light Scattering : With Applications to Chemistry, Biology, and Phys. (Dover Publications; Unabridged edition (August 14, 2000) 2000).

10.       Yariv, Amnon, and Pochi Yeh. Optical waves in crystals. (Vol. 5. New York: Wiley, 1984.)

11.        N. Bloembergen Nonlinear optics; a lecture note and reprint volume (W.A. Benjamin, New York,, 1965).

12.       R. W. Boyd Nonlinear optics (Academic Press, Amsterdam ; Boston, 2008).

13.       Y. R. Shen The principles of nonlinear optics (J. Wiley, New York, 1984).

14.       B. R. Masters and P. T. C. So Handbook of biomedical nonlinear optical microscopy (Oxford University Press, New York, 2008).