Spring 2016

ECE 564 Modern Light Microscopy

Prof. Gabriel Popescu

 

Text: “Introduction to Optical Microscopy” by J. Mertz (Roberts and Company, 2010)

“Quantitative phase imaging of cells and tissues” by G. Popescu (McGraw-Hill, 2011)

Schedule: 11:00-12:20 T-R, 3020 ECE Building;

Teaching Assistant:

Office Hours: Thursday 12:30-2:00, 4055 Beckman

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Research Projects

#

Day

Date

Topic

Supporting Material

Homework

Links

References

28

T

Jan 19

Introduction

Microscopy Milestones (Nature)

ECE 460

27

R

Jan 21

Groundwork: 2D, 3D Fourier transforms

Groundwork

ImageJ

Papoulis [1], Bracewell [2], Goodman [3]

26

T

Jan 26

Groundwork: 2D, 3D Fourier transforms

 

HW 1

25

R

Jan 28

Light Microscopy: ECE 460 review

Microscopy

 

 

Popescu [4]

24

T

Feb 02

Light Microscopy: ECE 460 review

 

HW2

 

 

23

R

Feb 04

Holography

Holography

 

 

 

22

T

Feb 9

Quantitative phase imaging (QPI)

QPI

 

 

 

21

R

Feb 11

Quantitative phase imaging (QPI)

 

HW3

 

 

20

T

Feb 16

Prof. Andrew Smith, Guest Lecture:

Nanoparticle Engineering

 

 

19

R

Feb 18

Propagation in inhomogeneous media

Light scattering

 

 

Born & Wolf [5], van de Hulst [6], Ishimaru [7]

18

T

Feb 23

Prof. Stephen Boppart, Guest Lecture:

Optical Coherence Tomography

 

 

 

17

R

Feb 25

Dynamic light scattering

Dynamic Light Scattering

HW4

 

Berne & Pecora [8]

16

T

Mar 01

Prof. Kimani Toussaint, Guest Lecture:

Nonlinear Microscopy

 

15

R

Mar 03

Propagation of Gaussian beams

Gaussian beams

HW5

 

Yariv- Optical waves in crystals

14

T

Mar 8

Propagation of Gaussian beams

 

HW6

 

 

13

R

Mar 10

Confocal microscopy

Confocal Microscopy

 

 

 

12

T

Mar 15

Review

 

 

R

Mar 17

Midterm

 

 

 

T

Mar 22

Spring Break

 

 

 

R

Mar 24

Spring Break

 

11

T

Mar 29

Optical coherence tomography

OCT

 

 

 

10

R

Mar 31

Optical coherence tomography

 

 

 

 

9

T

Apr 05

Propagation in nonlinear media

Nonlinear

 

 

Bloembergen [9], Boyd [10], Shen [11]

8

R

Apr 7

Propagation in nonlinear media

 

7

T

Apr 12

Nonlinear microscopy

 

Masters and So [12]

6

R

Apr 14

Nonlinear microscopy

Research Projects

5

T

Apr 19

Superresolution microscopy

 

 

 

4

R

Apr 21

Superresolution microscopy

Presentation Template

 

 

3

T

Apr 26

Student TALKS

 

 

2

R

Apr 28

Student TALKS

Peer evaluation form

 

1

T

May 03

Student TALKS

 

 

R

May 5

Reading Day

 

 

R

May 5

Reports due by email; by 5PM May 5

Report Template.

 

 

Grading formula: Midterm 30%;  Final report - 20%;  Homework - 30%;  Presentation 20%; Class participation - 5% .

 

REFERENCES

1.         A. Papoulis The Fourier integral and its applications (McGraw-Hill, New York,, 1962).

2.         R. N. Bracewell The Fourier transform and its applications (McGraw Hill, Boston, 2000).

3.         J. W. Goodman Introduction to Fourier optics (McGraw-Hill, New York, 1996).

4.         G. Popescu Quantitative phase imaging of cells and tissues (McGraw-Hill, New York, 2011).

5.         M. Born and E. Wolf Principles of optics : electromagnetic theory of propagation, interference and diffraction of light (Cambridge University Press, Cambridge ; New York, 1999).

6.         H. C. van de Hulst Light Scattering by Small Particles (Dover Publications New York, 1981).

7.         A. Ishimaru Electromagnetic wave propagation, radiation, and scattering (Prentice Hall, Englewood Cliffs, N.J., 1991).

8.         B. J. Berne and R. Pecora Dynamic Light Scattering : With Applications to Chemistry, Biology, and Phys. (Dover Publications; Unabridged edition (August 14, 2000) 2000).

9.         N. Bloembergen Nonlinear optics; a lecture note and reprint volume (W.A. Benjamin, New York,, 1965).

10.       R. W. Boyd Nonlinear optics (Academic Press, Amsterdam ; Boston, 2008).

11.       Y. R. Shen The principles of nonlinear optics (J. Wiley, New York, 1984).

12.       B. R. Masters and P. T. C. So Handbook of biomedical nonlinear optical microscopy (Oxford University Press, New York, 2008).