Spring 2015

ECE 564 Modern Light Microscopy

Prof. Gabriel Popescu

 

Text: “Introduction to Optical Microscopy” by J. Mertz (Roberts and Company, 2010)

“Quantitative phase imaging of cells and tissues” by G. Popescu (McGraw-Hill, 2011)

Schedule: 11:00-12:20 T-R, 3020 ECE Building;

Teaching Assistant: Mikhail Kandell (email)

Office Hours: Thursday 12:30-2:00, 4055 Beckman

Description: Description: Description: Description: Description: Description: Description: Description: subplot_small.gif

Research Projects

#

Day

Date

Topic

Supporting Material

Homework

Links

References

28

T

Jan 20

Introduction

Microscopy Milestones (Nature)

ECE 460

27

R

Jan 22

Groundwork: 2D, 3D Fourier transforms

Groundwork

ImageJ

Papoulis [1], Bracewell [2], Goodman [3]

26

T

Jan 27

Groundwork: 2D, 3D Fourier transforms

 

HW 1

25

R

Jan 29

Light Microscopy: ECE 460 review

Microscopy

 

 

Popescu [4]

24

T

Feb 03

Light Microscopy: ECE 460 review

 

HW2

 

 

23

R

Feb 05

Holography

Holography

 

 

 

22

T

Feb 10

Quantitative phase imaging (QPI)

QPI

 

 

 

21

R

Feb 12

Quantitative phase imaging (QPI)

 

HW3

 

 

20

T

Feb 17

Propagation in inhomogeneous media

Light scattering

 

Born & Wolf [5], van de Hulst [6], Ishimaru [7]

19

R

Feb 19

Propagation in inhomogeneous media

 

 

 

 

18

T

Feb 24

Propagation in inhomogeneous media

 

HW4

 

 

17

R

Feb 26

Dynamic light scattering

Dynamic Light Scattering

 

 

Berne & Pecora [8]

16

T

Mar 03

Dynamic light scattering

 

HW5

15

R

Mar 05

Propagation of Gaussian beams

Gaussian beams

 

 

Yariv- Optical waves in crystals

14

T

Mar 10

Propagation of Gaussian beams

 

HW6

 

 

13

R

Mar 12

Confocal microscopy

Confocal Microscopy

 

 

 

12

T

Mar 17

Review

 

 

R

Mar 19

Midterm

 

 

 

T

Mar 24

Spring Break

 

 

 

R

Mar 26

Spring Break

 

11

T

Mar 31

Optical coherence tomography

OCT

 

 

 

10

R

Apr 02

Optical coherence tomography

 

 

 

 

9

T

Apr 07

Propagation in nonlinear media

Nonlinear

 

 

Bloembergen [9], Boyd [10], Shen [11]

8

R

Apr 9

Propagation in nonlinear media

 

7

T

Apr 14

Nonlinear microscopy

 

Masters and So [12]

6

R

Apr 16

Nonlinear microscopy

Research Projects

5

T

Apr 21

Superresolution microscopy

 

 

 

4

R

Apr 23

Superresolution microscopy

Presentation Template

 

 

3

T

Apr 28

Student TALKS

 

 

2

R

Apr 30

Student TALKS

Peer evaluation form

 

1

T

May 05

Student TALKS

 

 

R

May 7

Reading Day

 

 

R

May 8

Reports due by email

Report Template.

 

 

Grading formula: Midterm 30%;  Final report - 20%;  Homework - 30%;  Presentation 20%; Class participation - 5% .

 

REFERENCES

1.         A. Papoulis The Fourier integral and its applications (McGraw-Hill, New York,, 1962).

2.         R. N. Bracewell The Fourier transform and its applications (McGraw Hill, Boston, 2000).

3.         J. W. Goodman Introduction to Fourier optics (McGraw-Hill, New York, 1996).

4.         G. Popescu Quantitative phase imaging of cells and tissues (McGraw-Hill, New York, 2011).

5.         M. Born and E. Wolf Principles of optics : electromagnetic theory of propagation, interference and diffraction of light (Cambridge University Press, Cambridge ; New York, 1999).

6.         H. C. van de Hulst Light Scattering by Small Particles (Dover Publications New York, 1981).

7.         A. Ishimaru Electromagnetic wave propagation, radiation, and scattering (Prentice Hall, Englewood Cliffs, N.J., 1991).

8.         B. J. Berne and R. Pecora Dynamic Light Scattering : With Applications to Chemistry, Biology, and Phys. (Dover Publications; Unabridged edition (August 14, 2000) 2000).

9.         N. Bloembergen Nonlinear optics; a lecture note and reprint volume (W.A. Benjamin, New York,, 1965).

10.       R. W. Boyd Nonlinear optics (Academic Press, Amsterdam ; Boston, 2008).

11.       Y. R. Shen The principles of nonlinear optics (J. Wiley, New York, 1984).

12.       B. R. Masters and P. T. C. So Handbook of biomedical nonlinear optical microscopy (Oxford University Press, New York, 2008).