Spring 2018

ECE 564 Modern Light Microscopy

Prof. Gabriel Popescu

 

Text: “Introduction to Optical Microscopy” by J. Mertz (Roberts and Company, 2010)

“Quantitative phase imaging of cells and tissues” by G. Popescu (McGraw-Hill, 2011)

Schedule: 11:00-12:20 T-R, 3020 ECE Building;

Office Hours: Thursday 12:30-2:00, 4055 Beckman

Teaching Assistant:

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Research Projects

#

Day

Date

Topic

Supporting Material

Homework

Links

References

28

T

Jan 16

Introduction

Microscopy Milestones (Nature)

ECE 460

27

R

Jan 18

Groundwork: 2D, 3D Fourier transforms

Groundwork

ImageJ

Papoulis [1], Bracewell [2], Goodman [3]

26

T

Jan 23

Groundwork: 2D, 3D Fourier transforms

 

25

R

Jan 25

Fourier Optics

Microscopy

 

 

Popescu [4]

24

T

Jan 30

Fourier Optics

 

 

 

23

R

Feb 1

Light Microscopy

Holography

 

 

 

22

T

Feb 6

Holography

QPI

 

 

 

21

R

Feb 8

Quantitative phase imaging (QPI)

 

 

 

20

T

Feb 13

Quantitative phase imaging (QPI)

 

19

R

Feb 15

Propagation in inhomogeneous media

Light scattering

 

 

Born & Wolf [5], van de Hulst [6], Ishimaru [7]

18

T

Feb 20

Propagation in inhomogeneous media

 

 

 

17

R

Feb 22

Dynamic light scattering

Dynamic Light Scattering

 

Berne & Pecora [8]

16

T

Feb 27

Dynamic light scattering

 

15

R

Mar 1

Propagation of Gaussian beams

Gaussian beams

 

Yariv- Optical waves in crystals

14

T

Mar 6

Propagation of Gaussian beams

 

 

 

13

R

Mar 8

Confocal microscopy

Confocal Microscopy

 

 

 

12

T

Mar 13

Review

 

 

R

Mar 15

Midterm

 

 

 

T

Mar 20

Spring Break

 

 

 

R

Mar 22

Spring Break

 

11

T

Mar 27

Optical coherence tomography

OCT

 

 

 

10

R

Mar 29

Optical coherence tomography

 

 

 

 

9

T

Apr 3

Propagation in nonlinear media

Nonlinear

 

 

Bloembergen [9], Boyd [10], Shen [11]

8

R

Apr 5

Propagation in nonlinear media

 

7

T

Apr 10

Nonlinear microscopy

 

Masters and So [12]

6

R

Apr 12

Nonlinear microscopy

Research Projects

5

T

Apr 17

Superresolution microscopy

 

 

 

4

R

Apr 19

Superresolution microscopy

Presentation Template

 

 

3

T

Apr 24

Student TALKS

 

 

2

R

Apr 26

Student TALKS

Peer evaluation form

 

1

T

May 1

Student TALKS

 

 

R

May 3

Reading Day

 

 

R

May 4

Reports due by email; by 5PM May 4

Report Template.

 

 

Grading formula: Midterm 30%;  Final report - 20%;  Homework - 30%;  Presentation 20%; Class participation - 5% .

 

REFERENCES

1.         A. Papoulis The Fourier integral and its applications (McGraw-Hill, New York,, 1962).

2.         R. N. Bracewell The Fourier transform and its applications (McGraw Hill, Boston, 2000).

3.         J. W. Goodman Introduction to Fourier optics (McGraw-Hill, New York, 1996).

4.         G. Popescu Quantitative phase imaging of cells and tissues (McGraw-Hill, New York, 2011).

5.         M. Born and E. Wolf Principles of optics : electromagnetic theory of propagation, interference and diffraction of light (Cambridge University Press, Cambridge ; New York, 1999).

6.         H. C. van de Hulst Light Scattering by Small Particles (Dover Publications New York, 1981).

7.         A. Ishimaru Electromagnetic wave propagation, radiation, and scattering (Prentice Hall, Englewood Cliffs, N.J., 1991).

8.         B. J. Berne and R. Pecora Dynamic Light Scattering : With Applications to Chemistry, Biology, and Phys. (Dover Publications; Unabridged edition (August 14, 2000) 2000).

9.         N. Bloembergen Nonlinear optics; a lecture note and reprint volume (W.A. Benjamin, New York,, 1965).

10.       R. W. Boyd Nonlinear optics (Academic Press, Amsterdam ; Boston, 2008).

11.       Y. R. Shen The principles of nonlinear optics (J. Wiley, New York, 1984).

12.       B. R. Masters and P. T. C. So Handbook of biomedical nonlinear optical microscopy (Oxford University Press, New York, 2008).