Spring 2014

ECE 564 Modern Light Microscopy

Prof. Gabriel Popescu

 

Text: “Introduction to Optical Microscopy” by J. Mertz (Roberts and Company, 2010)

“Quantitative phase imaging of cells and tissues” by G. Popescu (McGraw-Hill, 2011)

Schedule: 11:00-12:20 T-R, 260 Everitt Lab;

Teaching Assistant:

Office Hours:

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Research Projects

#

Day

Date

Topic

Supporting Material

Homework

Links

References

28

T

Jan 21

Introduction

Microscopy Milestones (Nature)

1D Fourier Transforms (ECE 460)

27

R

Jan 23

Groundwork: 2D, 3D Fourier transforms

Groundwork

ImageJ

Papoulis [1], Bracewell [2], Goodman [3]

26

T

Jan 28

Groundwork: 2D, 3D Fourier transforms

 

HW 1

25

R

Jan 30

Light Microscopy: ECE 460 review

Microscopy

 

 

 

24

T

Feb 04

Light Microscopy: ECE 460 review

 

HW2

 

 

23

R

Feb 06

Holography

Holography

 

 

 

22

T

Feb 11

Quantitative phase imaging (QPI)

QPI

 

 

 

21

R

Feb 13

Quantitative phase imaging (QPI)

 

HW3

 

 

20

T

Feb 18

Propagation in inhomogeneous media

Light scattering

 

Born & Wolf [4], van de Hulst [6], Ishimaru [7]

19

R

Feb 20

Propagation in inhomogeneous media

 

 

 

 

18

T

Feb 25

Propagation in inhomogeneous media

 

HW4

 

 

17

R

Feb 27

Dynamic light scattering

Dynamic Light Scattering

 

 

Berne & Pecora [8]

16

T

Mar 04

Dynamic light scattering

 

HW5

15

R

Mar 06

Propagation of Gaussian beams

Gaussian beams

 

 

Yariv- Optical waves in crystals

14

T

Mar 11

Propagation of Gaussian beams

 

HW6

 

 

13

R

Mar 13

Confocal microscopy

Confocal Microscopy

 

 

 

12

T

Mar 18

Review

 

 

R

Mar 20

Midterm

 

 

 

T

Mar 25

Spring Break

 

 

 

R

Mar 27

Spring Break

 

11

T

Apr 01

Optical coherence tomography

OCT

 

 

 

10

R

Apr 03

Optical coherence tomography

 

 

 

 

9

T

Apr 08

Propagation in nonlinear media

Nonlinear

 

 

Bloembergen [9], Boyd [10], Shen [11]

8

R

Apr 10

Propagation in nonlinear media

 

7

T

Apr 15

Nonlinear microscopy

 

6

R

Apr 17

Nonlinear microscopy

Research Projects

5

T

Apr 22

Superresolution microscopy

 

 

 

4

R

Apr 24

Superresolution microscopy

Presentation Template

 

 

3

T

Apr 29

Student TALKS

 

 

2

R

May 01

Student TALKS

Peer evaluation form

 

1

T

May 06

Student TALKS

 

 

R

May 8

Reading Day

 

 

R

May 9

Reports due by email

Report Template.

 

 

Grading formula: Midterm 30%;  Final report - 20%;  Homework - 30%;  Presentation 20%; Class participation - 5% .

 

REFERENCES

[1]           A. Papoulis, The Fourier integral and its applications. New York,: McGraw-Hill, 1962.

[2]           R. N. Bracewell, The Fourier transform and its applications, 3rd ed. Boston: McGraw Hill, 2000.

[3]           J. W. Goodman, Introduction to Fourier optics, 2nd ed. New York: McGraw-Hill, 1996.

[4]           M. Born and E. Wolf, Principles of optics : electromagnetic theory of propagation, interference and diffraction of light, 7th expanded ed. Cambridge ; New York: Cambridge University Press, 1999.

[5]           J. D. Jackson, Classical electrodynamics, 3rd ed. New York: Wiley, 1999.

[6]           H. C. van de Hulst, Light Scattering by Small Particles New York: Dover Publications 1981.

[7]           A. Ishimaru, Electromagnetic wave propagation, radiation, and scattering. Englewood Cliffs, N.J.: Prentice Hall, 1991.

[8]           B. J. Berne and R. Pecora, Dynamic Light Scattering : With Applications to Chemistry, Biology, and Phys. : Dover Publications; Unabridged edition (August 14, 2000) 2000.

[9]           N. Bloembergen, Nonlinear optics; a lecture note and reprint volume. New York,: W.A. Benjamin, 1965.

[10]         R. W. Boyd, Nonlinear optics, 3rd ed. Amsterdam ; Boston: Academic Press, 2008.

[11]         Y. R. Shen, The principles of nonlinear optics. New York: J. Wiley, 1984.

[12]         L. Mandel and E. Wolf, "Coherence Properties of Optical Fields," Reviews of Modern Physics, vol. 37, p. 231, 1965.

[13]         L. Mandel and E. Wolf, Optical coherence and quantum optics. Cambridge ; New York: Cambridge University Press, 1995.

[14]         R. J. Glauber, "The quantum theory of optical coherence," Physical Review, vol. 130, p. 2529, 1963.

[15]         J. W. Goodman, Statistical optics. New York: Wiley, 1985.

[16]         M. Pluta, Advanced light microscopy. Warszawa: Polish Scientific Publishers, 1988.