Spring 2017

ECE 564 Modern Light Microscopy

Prof. Gabriel Popescu

 

Text: “Introduction to Optical Microscopy” by J. Mertz (Roberts and Company, 2010)

“Quantitative phase imaging of cells and tissues” by G. Popescu (McGraw-Hill, 2011)

Schedule: 11:00-12:20 T-R, 3020 ECE Building;

Teaching Assistant:

Office Hours: Thursday 12:30-2:00, 4055 Beckman

Description: Description: Description: Description: Description: Description: Description: Description: subplot_small.gif

Research Projects

#

Day

Date

Topic

Supporting Material

Homework

Links

References

28

T

Jan 17

Introduction

Microscopy Milestones (Nature)

ECE 460

27

R

Jan 19

Groundwork: 2D, 3D Fourier transforms

Groundwork

ImageJ

Papoulis [1], Bracewell [2], Goodman [3]

26

T

Jan 24

Groundwork: 2D, 3D Fourier transforms

 

HW 1

25

R

Jan 26

Fourier Optics

Microscopy

 

 

Popescu [4]

24

T

Jan 31

Fourier Optics

 

HW2

 

 

23

R

Feb 2

Light Microscopy

Holography

 

 

 

22

T

Feb 7

Holography

QPI

 

 

 

21

R

Feb 9

Quantitative phase imaging (QPI)

 

 

 

20

T

Feb 14

Quantitative phase imaging (QPI)

HW3

 

19

R

Feb 16

Propagation in inhomogeneous media

Light scattering

 

 

Born & Wolf [5], van de Hulst [6], Ishimaru [7]

18

T

Feb 21

Propagation in inhomogeneous media

 

HW4

 

 

17

R

Feb 23

Dynamic light scattering

Dynamic Light Scattering

 

Berne & Pecora [8]

16

T

Feb 28

Dynamic light scattering

 

HW5

15

R

Mar 02

Propagation of Gaussian beams

Gaussian beams

 

Yariv- Optical waves in crystals

14

T

Mar 7

Propagation of Gaussian beams

 

HW6

 

 

13

R

Mar 9

Confocal microscopy

Confocal Microscopy

 

 

 

12

T

Mar 14

Review

 

 

R

Mar 16

Midterm

 

 

 

T

Mar 21

Spring Break

 

 

 

R

Mar 23

Spring Break

 

11

T

Mar 28

Optical coherence tomography

OCT

 

 

 

10

R

Mar 30

Optical coherence tomography

 

 

 

 

9

T

Apr 4

Propagation in nonlinear media

Nonlinear

 

 

Bloembergen [9], Boyd [10], Shen [11]

8

R

Apr 6

Propagation in nonlinear media

 

7

T

Apr 11

Nonlinear microscopy

 

Masters and So [12]

6

R

Apr 13

Nonlinear microscopy

Research Projects

5

T

Apr 18

Superresolution microscopy

 

 

 

4

R

Apr 20

Superresolution microscopy

Presentation Template

 

 

3

T

Apr 25

Student TALKS

 

 

2

R

Apr 27

Student TALKS

Peer evaluation form

 

1

T

May 02

Student TALKS

 

 

R

May 4

Reading Day

 

 

R

May 5

Reports due by email; by 5PM May 5

Report Template.

 

 

Grading formula: Midterm 30%;  Final report - 20%;  Homework - 30%;  Presentation 20%; Class participation - 5% .

 

REFERENCES

1.         A. Papoulis The Fourier integral and its applications (McGraw-Hill, New York,, 1962).

2.         R. N. Bracewell The Fourier transform and its applications (McGraw Hill, Boston, 2000).

3.         J. W. Goodman Introduction to Fourier optics (McGraw-Hill, New York, 1996).

4.         G. Popescu Quantitative phase imaging of cells and tissues (McGraw-Hill, New York, 2011).

5.         M. Born and E. Wolf Principles of optics : electromagnetic theory of propagation, interference and diffraction of light (Cambridge University Press, Cambridge ; New York, 1999).

6.         H. C. van de Hulst Light Scattering by Small Particles (Dover Publications New York, 1981).

7.         A. Ishimaru Electromagnetic wave propagation, radiation, and scattering (Prentice Hall, Englewood Cliffs, N.J., 1991).

8.         B. J. Berne and R. Pecora Dynamic Light Scattering : With Applications to Chemistry, Biology, and Phys. (Dover Publications; Unabridged edition (August 14, 2000) 2000).

9.         N. Bloembergen Nonlinear optics; a lecture note and reprint volume (W.A. Benjamin, New York,, 1965).

10.       R. W. Boyd Nonlinear optics (Academic Press, Amsterdam ; Boston, 2008).

11.       Y. R. Shen The principles of nonlinear optics (J. Wiley, New York, 1984).

12.       B. R. Masters and P. T. C. So Handbook of biomedical nonlinear optical microscopy (Oxford University Press, New York, 2008).