ECE564: MODERN LIGHT MICROSCOPY

Research Projects

Students will prepare a written report (report_template.doc) and will give a PowerPoint presentation (presentation_template.ppt) on a subject from the list below.

Topic	Supporting material	Student in charge--Schedule
SUPERRESOLUTION IMAGING
1. Near-field scanning optical microscopy (NSOM)	Science 1992, Betzig	Joseph Tibbs----April 27, 2021
2. Stimulated emission depletion microscopy (STED)	OL 1994, Hell	Priyash Barya--April 27, 2021
3. Nonlinear structured illumination microscopy	PNAS 2005, Gustaffson	Zhongmin Zhu--April 27, 2021
4. Super-localization: STORM/PALM/fPALM	Nat. Meth. 2006, Zhuang , Science 2006, Betzig, Biophys. J. 2006, Hess	Abigail Berg--April 29, 2021
5. Super-resolution optical fluctuation imaging (SOFI)	PNAS 2009, Dertinger
NONLINEAR MICROSCOPY
6. Two photon fluorescence microscopy (TPFM)	Review article, So et al.	Bing Ze Lin--April 29, 2021
7. Second harmonic generation microscopy (SHGM)	PNAS 2003, Webb Group	Justin Haque---April 29, 2021
8. Coherent anti-Stokes Raman scattering (CARS)	PRL 1999, Xie Group	Swetapadma Sahoo---May 04, 2021
FLUORESCENCE TECHNIQUES
9. Green fluorescence protein (GFP)	Review 1998, Tsien
10. Fluorescence resonance energy transfer (FRET)	Science review, Tsien Group	Skye Shepherd---May 04, 2021
11. Deconvolution microscopy	Review 1999, McNally et al.
12. Total internal reflection fluorescence (TIRF) microscopy	FRAP, review
13. Fluorescence Lifetime Imaging Microscopy (FLIM)	Review, Clegg	Yen-Ting Liu---May 04, 2021
14. Optogenetics	Review, Fenno	Han Keun Lee---May 10, 2021
15. Light sheet microscopy (LSM)	Science 2008, Keller et al.
16. Lattice light-sheet microscopy	Science 2014, Chen et al.
LABEL-FREE METHODS
17. Interferometric synthetic aperture microscopy (ISAM)	Nat. Phys. 2007, Ralston et al.
18. Diffraction phase microscopy (DPM)	Review 2014, Bhaduri et al	Thomas Reboli---May 10, 2021
19. Digital holographic microscopy (DHM)	Review 2014, Marquet et al.	Lawrence Ju---May 10, 2021
20. Diffuse optical tomography (DOT)	PNAS 2000, Ntziachristos	Alexander Littlefield---May 10, 2021
21. Fourier Transform Infrared Spectroscopy of tissues	Review 2008, Movasaghi
22. Stimulated Raman scattering (SRS)	Science 2008, Freudiger
23. Surface enhanced Raman scattering (SERS)	Review 1998, Campion	Liuqing Gao---May 10, 2021
24. Photoacoustic microscopy (PAM)	PAM- Nature Biotech
DYNAMICS
25. Fluorescence recovery after photobleaching (FRAP)	Review, Axelrod	Shensheng Zhao---May 10, 2021
26. Fluorescence correlation spectroscopy (FCS)	Review 2002, Krichevsky	Zuodong Liang---May 10, 2021
27. Fluorescence speckle microscopy (FSM)	Review 2006, Danuser	Brendan Wolan----May 10, 2021
28. Diffusing wave spectroscopy (DWS)	PRL 1988, Pine
29. Microrheology	Review 2005, Waigh	Pin-Chiao Huang---May 10, 2021
30. Traction force microscopy	Review 2014, Plotnikov