ECE564:  MODERN LIGHT MICROSCOPY

Research Projects

 

Students will prepare a written report (report_template.doc) and will give a PowerPoint presentation (presentation_template.ppt) on a subject from the list below.

Topic

Supporting material

Student in charge

SUPERRESOLUTION IMAGING

 

 

1.      Near-field scanning optical microscopy (NSOM)

Science 1992, Betzig

 

2.      Stimulated emission depletion microscopy (STED)

OL 1994, Hell

 

3.      Nonlinear structured illumination microscopy

PNAS 2005, Gustaffson

 

4.      Super-localization: STORM/PALM/fPALM

Nat. Meth. 2006, Zhuang , Science 2006, Betzig, Biophys. J. 2006, Hess

 

5.      Super-resolution optical fluctuation imaging (SOFI)

PNAS 2009, Dertinger

 

NONLINEAR MICROSCOPY

 

 

6.      Two photon fluorescence microscopy (TPFM)

Review article, So et al.

 

7.      Second harmonic generation microscopy (SHGM)

PNAS 2003, Webb Group

 

8.      Coherent anti-Stokes Raman scattering (CARS)

PRL 1999, Xie Group

 

FLUORESCENCE TECHNIQUES

9.      Green fluorescence protein (GFP)

Review 1998, Tsien

10.   Fluorescence resonance energy transfer (FRET)

Science review, Tsien Group

11.   Deconvolution microscopy

Review 1999, McNally et al.

 

12.   Total internal reflection fluorescence (TIRF) microscopy

FRAP, review

13.   Fluorescence Lifetime Imaging Microscopy (FLIM)

Review, Clegg

14.   Optogenetics

Review, Fenno

 

15.   Light sheet microscopy (LSM)

Science 2008, Keller et al.

 

16.   Lattice light-sheet microscopy

Science 2014, Chen et al.

LABEL-FREE METHODS

 

 

17.   Interferometric synthetic aperture microscopy (ISAM)

Nat. Phys. 2007, Ralston et al.

 

18.   Diffraction phase microscopy (DPM)

Review 2014, Bhaduri et al

 

19.   Digital holographic microscopy (DHM)

Review 2014, Marquet et al.

 

20.   Diffuse optical tomography (DOT)

PNAS 2000, Ntziachristos

 

21.   Fourier Transform Infrared Spectroscopy of tissues

Review 2008, Movasaghi

 

22.   Stimulated Raman scattering (SRS)

Science 2008, Freudiger

 

23.   Surface enhanced Raman scattering (SERS)

Review 1998, Campion

 

24.   Photoacoustic microscopy (PAM)

PAM- Nature Biotech

DYNAMICS

 

 

25.   Fluorescence recovery after photobleaching (FRAP)

Review, Axelrod

26.   Fluorescence correlation spectroscopy (FCS)

Review 2002, Krichevsky

 

27.   Fluorescence speckle microscopy (FSM)

Review 2006, Danuser

 

28.   Diffusing wave spectroscopy (DWS)

PRL 1988, Pine

 

29.   Microrheology

Review 2005, Waigh

 

30.   Traction force microscopy

 

Review 2014, Plotnikov