ECE564: MODERN LIGHT MICROSCOPY

Research Projects

Students will prepare a written report (report_template.doc) and will give a PowerPoint presentation (presentation_template.ppt) on a subject from the list below.

Topic	Supporting material	Student in charge
FLUORESCENCE TECHNIQUES
1. Green fluorescence protein (GFP)	Review 1998, Tsien
2. Fluorescence resonance energy transfer (FRET)	Science review, Tsien Group
3. Total internal reflection fluorescence (TIRF) microscopy	Review, Axelrod
4. Fluorescence recovery after photobleaching (FRAP)	FRAP, review
5. Fluorescence Lifetime Imaging Microscopy (FLIM)	Review, Clegg
6. Fluorescence correlation spectroscopy (FCS)	Review 2002, Krichevsky
NONLINEAR MICROSCOPY
7. Two photon fluorescence microscopy (TPFM)	Review article, So et al.
8. Second harmonic generation microscopy (SHGM)	PNAS 2003, Webb Group
9. Coherent anti-Stokes Raman scattering (CARS) microscopy	PRL 1999, Xie Group
NANOSCOPY	2009 Nanoscopy review, Hell
10. Near-field scanning optical microscopy (NSOM)	Science 1992, Betzig
11. Stimulated emission depletion microscopy (STED)	OL 1994, Hell
12. Nonlinear structured illumination microscopy	PNAS 2005, Gustaffson
13. Super-localization: STORM/PALM/fPALM	Nat. Meth. 2006, Zhuang Group, Science 2006, Betzig, Biophys. J. 2006, Hess
OTHERS
14. Photoacoustic microscopy (PAM)	PAM- Nature Biotech