Spring 2021
ECE 564 Modern Light Microscopy
Text: “Introduction to Optical Microscopy” by J. Mertz (Roberts and Company, 2010)
“Quantitative phase imaging of cells and tissues” by G. Popescu (McGrawHill, 2011)
Schedule: 11:0012:20 TR;
Office Hours: Thursday 12:302:00
Teaching Assistant: Neha Goswami (email)
TA Office Hours: Friday 3:004:00pm
# 
Day 
Date 
Topic 
Supporting Material 
Homework 
Links 
References 
28 
T 
Jan 26 
Introduction 

27 
R 
Jan 28 
Groundwork: 2D, 3D Fourier transforms 

26 
T 
Feb 02 
Groundwork: 2D, 3D Fourier transforms 


25 
R 
Feb 04 
Fourier Optics 


Popescu [5] 

24 
T 
Feb 09 
Fourier Optics 




23 
R 
Feb 11 
Light Microscopy 




22 
T 
Feb 16 
Holography 




21 
R 
Feb 18 
Quantitative phase imaging (QPI) 




20 
T 
Feb 23 
Quantitative phase imaging (QPI) 


19 
R 
Feb 25 
Propagation in inhomogeneous media 



18 
T 
Mar 02 
Propagation in inhomogeneous media 




17 
R 
Mar 04 
Dynamic light scattering 

Berne & Pecora [9] 

16 
T 
Mar 09 
Dynamic light scattering 


15 
R 
Mar 11 
Propagation of Gaussian beams 

Yariv Optical waves in crystals [10] 

14 
T 
Mar 16 
Propagation of Gaussian beams 




13 
R 
Mar 18 
Confocal microscopy 




12 
T 
Mar 23 
Review 



R 
Mar 25 
Midterm 



11 
T 
Mar 30 
Optical coherence tomography 




10 
R 
Apr 01 
Optical coherence tomography 




9 
T 
Apr 06 
Propagation in nonlinear media 



8 
R 
Apr 08 
Propagation in nonlinear media 


T 
Apr 13 
Day off 



7 
R 
Apr 15 
Nonlinear microscopy 

Masters and So [14]  
6 
T 
Apr 20 
Nonlinear microscopy 




5 
R 
Apr 22 
Superresolution microscopy 




T 
Apr 27 
Student TALKS 




R 
Apr 29 
Student TALKS 
Peer evaluation form 


2 
T 
May 04 
Student TALKS 



R 
May 06 
Reading Day 



1 
M 
May 10 
Student TALKS 



M 
May 10 
Reports due by midnight May 10. Upload to your Box folder 
Report Template. 

Grading formula: Midterm 30%; Final report  20%; Homework  30%; Presentation 20%; Class participation  5% .
REFERENCES
1. A. Papoulis The Fourier integral and its applications (McGrawHill, New York,, 1962).
2. R. N. Bracewell The Fourier transform and its applications (McGraw Hill, Boston, 2000).
3. J. W. Goodman Introduction to Fourier optics (McGrawHill, New York, 1996).
5. G. Popescu Quantitative phase imaging of cells and tissues (McGrawHill, New York, 2011).
7. H. C. van de Hulst Light Scattering by Small Particles (Dover Publications New York, 1981).
10. Yariv, Amnon, and Pochi Yeh. Optical waves in crystals. (Vol. 5. New York: Wiley, 1984.)
12. R. W. Boyd Nonlinear optics (Academic Press, Amsterdam ; Boston, 2008).
13. Y. R. Shen The principles of nonlinear optics (J. Wiley, New York, 1984).