Matthew Schulmerich, May 2nd, 2011
Spatially off-set and transmission Raman spectroscopy, imaging, and tomography in light scattering media: applications towards the quantitative analysis of bone, prostate tissue, and economically important Soybean components
Raman spectroscopy has proven to be a non-destructive approach to obtaining chemically specific information from a wide variety of analytes.Conventional experimental configurations illuminate the sample and collect the backscattered Raman signal from the same points of illumination. However, by offsetting the illumination and collection regions, it is possible to tune the sampling depths to obtain signal from targets buried below the surface of light scattering materials.In this presentation a number of experimental examples will be presented illustrating applications for both spatially offset Raman spectroscopy and transmission Raman spectroscopy.We illustrate the ability to gain spectral/chemical information with both spatial and depth resolution using polymer models, tissue phantoms as well as murine and human tissue.Considerations toward quantitative measurements will be illustrated using prostate tissue and whole soybeans.