Archive: January, 2017

Refractive index variance of cells and tissues measured by quantitative phase imaging

Posted on 01/23/17 by Chenfei Hu

PDF Link The refractive index distribution of cells and tissues governs their interaction with light and can report on morphological modifications associated with disease. Through intensity-based measurements, refractive index information can be extracted only via scattering models that approximate light propagation. As a result, current knowledge of refractive index distributions across various tissues and cell […]

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White-light diffraction phase microscopy at doubled space-bandwidth product

Posted on 01/09/17 by Mikhail Kandel

PDF Link White light diffraction microscopy (wDPM) is a quantitative phase imaging method that benefits from both temporal and spatial phase sensitivity, granted, respectively, by the common-path geometry and white light illumination. However, like all off-axis quantitative phase imaging methods, wDPM is characterized by a reduced space-bandwidth product compared to phase shifting approaches. This happens […]

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Label-Free Imaging of Single Microtubule Dynamics Using Spatial Light Interference Microscopy

Posted on 01/09/17 by Mikhail Kandel

PDF Link Due to their diameter, of only 24 nm, single microtubules are extremely challenging to image without the use of extrinsic contrast agents. As a result, fluorescence tagging is the common method to visualize their motility. However, such investigation is limited by photobleaching and phototoxicity. We experimentally demonstrate the capability of combining label-free spatial […]

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Label-free, multi-scale imaging of ex-vivo mouse brain using spatial light interference microscopy

Posted on 01/09/17 by Mikhail Kandel

PDF Link Brain connectivity spans over broad spatial scales, from nanometers to centimeters. In order to understand the brain at multi-scale, the neural network in wide-field has been visualized in detail by taking advantage of light microscopy. However, the process of staining or addition of fluorescent tags is commonly required, and the image contrast is […]

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