Archive: December, 2014

Epi-illumination diffraction phase microscopy with white light

Posted on 12/09/14 by Taewoo

PDF Link We demonstrate the first reflection-based epi-illumination diffraction phase microscope with white light (epi-wDPM). The epi-wDPM system combines the off-axis, common-path, and white light approaches, in a reflection geometry enabling sub-nanometer spatial and temporal noise levels, while providing single-shot acquisition for opaque samples. We verified the epi-wDPM results by measuring control samples with known […]

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Quantitative Phase Imaging of Cells in 2-, 3- and 4-D

Posted on 12/09/14 by Taewoo

PDF Link Living cells are highly transparent under visible illumination, which limits our ability to study them without disruption. Zernike’s phase contrast microcopy has revolutionized cell biology by rendering intrinsic contrast from unlabeled cells. However, phase contrast is a qualitative method for visualization. Advances made in image sensors and computing technologies gave birth to a new […]

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In situ measurements of the axial expansion of palladium microdisks during hydrogen exposure using diffraction phase microscopy

Posted on 12/01/14 by Taewoo

PDF Link We measured the height of Pd microdisks during H2 exposure using epi-illumination diffraction phase microscopy, a quantitative phase imaging technique for capturing nanoscale dynamics in situ. From these microdisk height measurements, we extracted the axial expansion coefficient as a function of H2 concentration as well as image sequences that show the instantaneous rate […]

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