Imaging Collagen Properties in the Uterosacral Ligaments of Women With Pelvic Organ Prolapse Using Spatial Light Interference Microscopy (SLIM)

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We studied collagen fiber organization in tissue affected by pelvic organ prolapse (POP) and compared it to asymptomatic controls. Both the control and POP tissue biopsies were prepared and measured by a highly sensitive quantitative phase imaging (QPI) system, called spatial light interference microscopy (SLIM). Combined with automatic image processing, this modality provides quantitative, high-throughput assessment of fiber morphology. We found the fiber orientation in prolapsed specimens is less homogeneous, indicating an abnormal organization of collagen in the extracellular matrix (ECM).

Chenfei Hu