T. Kim and G. Popescu, Laplace field microscopy for label-free imaging of dynamic biological structures, Opt. Lett., 36(23), 2011

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We present Laplace field microscopy as a method for generating intrinsic contrast of transparent specimens. This technique uses a spatial light modulator to perform the Laplacian of the field in the Fourier plane of a microscope image. The resulting image incorporates phase information and thus render high contrast images from phase objects. We demonstrate the potential of the method by imaging index-matched beads, unlabeled tissue slices, and dynamic live cells.

Taewoo
Tae Woo Kim Graduate Research Assistant Electrical and Computer Engineering Quantitative Light Imaging Laboratory 4053 Beckman Institute University of Illinois, Urbana-Champaign tkim44@illinois.edu