T. Kim and G. Popescu, Laplace field microscopy for label-free imaging of dynamic biological structures, Opt. Lett., 36(23), 2011

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We present Laplace field microscopy as a method for generating intrinsic contrast of transparent specimens. This technique uses a spatial light modulator to perform the Laplacian of the field in the Fourier plane of a microscope image. The resulting image incorporates phase information and thus render high contrast images from phase objects. We demonstrate the potential of the method by imaging index-matched beads, unlabeled tissue slices, and dynamic live cells.

Tae Woo Kim Graduate Research Assistant Electrical and Computer Engineering Quantitative Light Imaging Laboratory 4053 Beckman Institute University of Illinois, Urbana-Champaign tkim44@illinois.edu